Questions_CellWall&Surface - Cell Wall and Surface...

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Unformatted text preview: Cell Wall and Surface Structures 1. G. stearothermophilus is a Gram-positive bacterium, that you find is able to specifically adhere to cellulose. You hypothesize that G. stearothermophilus produces a protein to that specifically binds cellulose and that is responsible for mediating binding of G. stearothermophilus cell to cellulose. In which subcellular structure/location would you expect the cellulose adhesion molecule be located? (Choose one) 1. Cytoplasm 4. Periplasm 2. Cytoplasmic membrane 5. Outer membrane 3. Peptidoglycan 2. You examine your G. stearothermophilus strains by transmission electron microscopy. You observe a structure outside of the presumed peptidoglycan layer that you hypothesize is a capsule. What test would you carry out to verify whether your G. stearothermophilus strain has a capsule? What result would you observe if your hypothesis is correct? 3. Cross-linking between peptides attached to peptidoglycan in bacterial cell walls requires a. any amino acid. b. ornithine. c. alanine. d. a diamino acid. e. a triamino acid. 4. What function has been attributed to peptidoglycan beside protection from turgor pressure? 5. Outline an experimental procedure to test that the S-layer on an Archaeal species is required for survival in low osmolarity. You can remove the S-layer with protease. Be sure to include a control in your experiment, and list the results you would expect if the S-layer were required for survival in low osmolarity. ...
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This note was uploaded on 03/06/2012 for the course MIMG 100 taught by Professor Lazazzera during the Summer '10 term at UCLA.

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