Questions for RNA Regulation

Questions for RNA Regulation - Homework for RNA...

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Unformatted text preview: Homework for RNA Regulation 1. How can a small, non ­coding RNA promote translation of a gene? 2. You find that nicotinamide activates expression of adpA by preventing attenuation. You hypothesize that that there is a nicotinamide riboswitch in the 5’ untranslated region of the adpA mRNA. Propose ONE experiment to test this hypothesis. Describe expected results if your hypothesis is correct. 3. In the presence of methonine in the growth medium, the metE gene of E. coli prematurely terminates transcription. In the absence of methionine, transcription of metE proceeds. You hypothesize that the untranslated region of the mRNA of metE encodes a riboswitch for methionine. Propose one experiment to test this hypothesis and the results you would obtain if your hypothesis is correct. 3. Expression of the proF gene is inhibited by proline. The mRNA for proF has a long, putatively untranslated region at the 5’ end that occurs before the translation start site for ProF. You make the proF lacZ transcriptional fusion as shown blow. You then make proF lacZ fusions with DNA deletions of portions of untranslated region of the 5’ end of the mRNA. You assay strains containing these proF lacZ transcriptional fusions during growth in medium without and with proline. The results are shown below. Propose a model to explain the effects of the different deletions on transcription of proF. ...
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