LECTURE _Notes2010S

LECTURE _Notes2010S - Introduction to the Microbial World...

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Unformatted text preview: Introduction to the Microbial World Part 1 of the Lecture This will be an introduction to the field of microbiology. Part 2 of the Lecture This is the part of the lecture that you will need to know for the exam: Pasteur’s experiment disproving spontaneous generation and Koch’s postulates. Textbook Reading Pages 39-45 Questions you should be able to answer after reading the text: 1. The observation was made, after food was left to stand for some time, that there would be a large number of microbes associated with it; whereas at the start, the food did not have a large number of microbes associated with it. Why can this observation be equally well explained by the theories of spontaneous generation of the microbes as well as microbes arose from pre-existing microbes? 2. Be able to outline Pasteur’s swan-neck flask experiment according the flow chart of the “Scientific Method” (see section below). 3. Did Pasteur’s experiment prove that spontaneous generation can never occur? 4. In Koch’s postulates, why is it important to show that the suspected pathogen can be re-isolated from the diseased animal? 5. In Koch’s postulates, why might an inoculated animal show disease and it not be possible to re-isolate the suspected pathogen? 1 Review of the Scientific Method Step 1: Observation Step 2: Hypothesis/Model/Theory Step 3: Design Experiment Experimental Group Control Groups Repeat from Step 2 Scientific hypotheses yield testable predictions. The experiments designed to test the hypothesis should yield one result (i.e. observation) if the hypothesis is correct and a different result if the hypothesis is incorrect. Control groups allow comparison between two samples that differ for a defined set of variables. 2 ...
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