{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

BIMM 120 Lecture11_ReadingGuide.050511

BIMM 120 Lecture11_ReadingGuide.050511 - Lecture 11...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 11 – BIMM120.SP11 LECTURE 10 – May 05, 2011 Metabolic Diversity I: Fermentation & Respiration (BLACK = this lecture / BLUE = different lecture / RED = not covered in lecture ) Chapter 5 – Nutrition, Culture, and Metabolism of Microorganisms (pp.122-125) IV. Essentials of Catabolism 5.9 Energy Conservation Substrate-Level Phosphorylation and Oxidative Phosphorylation o In fermentation, ATP is produced by substrate-level phosphorylation o In oxidative phosphorylation ATP is produced at the expense of the proton motive force 5.10 Glycolysis as an Example of Fermentation Stage I: Preparatory Reactions o Glucose is phosphorylated by ATP o Fructose 16 biphosphate is split into two 3-carbon molecules Stage II: The Production of NADH, ATP, and Pyruvate o Glyceraldehyde 3-phosphate is oxidized o NAD + is reduced to NADH o Pyruvate and ATP are produced Stage III: Consumption of NADH and the Production of Fermentation Products o The NADH produced in glycolysis must be oxidized back to NAD + in order for glycolysis to continue Glucose Fermentation: Net and Practical Results o During glycolysis glucose is consumed, two ATPs are made, and different fermentation products are generated Microbial Sidebar: The Products of Yeast Fermentation & the Pasteur Effect (pg 125) Chapter 21 – Metabolic Diversity: Catabolism of Organic Compounds (pp.613-618; 622-630) I. Fermentation 21.1 Fermentations: Energetic and Redox Considerations Fermentations o Many microbial habitats are anoxic o If adequate supplies of electron acceptors such as NO 3 - , Fe 3+ , and others are not available in anoxic microbial habitats, organic compounds are catabolized by fermentation o In fermentation, ATP synthesis usually occurs by substrate-level phosphorylation Energy-Rich Compounds and Substrate-Level Phosphorylation 1
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Lecture 11 – BIMM120.SP11 o Energy conservation by substrate-level phosphorylation occurs in many different ways o Central to ATP synthesis is the production of energy-rich compounds . These are organic compounds that contain an energy-rich phosphate bond or a molecule of coenzyme-A Oxidation-Reduction Balance, H 2 , and Acetate Production o There must be an oxidation-reduction (redox) balance in any fermentation o In a number of fermentations, electron balance is maintained by the production of molecular hydrogen (H 2 ) o The production of H 2 is associated with the activity of an iron-sulfur protein called ferredoxin o The transfer of electrons from ferredoxin to H + is catalyzed by the enzyme hydrogenase o
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}