BIMM 120 Lecture16_ReadingGuide.052411

BIMM 120 Lecture16_ReadingGuide.052411 - Lecture 16...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 16 – BIMM120.SP11 LECTURE 16 – May 24, 2011 Metagenomics: Sampling the Unknown (BLACK = this lecture / BLUE = different lecture / RED = not covered in lecture ) Chapter 22 – Methods in Microbial Ecology (pp. 658-662; 665-666) II. Culture-Independent Analyses of Microbial Communities 22.3 General Staining Methods Fluorescent Staining Using DAPI or Acridine Orange o Fluorescent dyes can be used to stain microorganisms from a microbial habitat. DAPI (4’, 6-diamido-2-phenylindole) is a popular stain for this purpose, as is the dye acridine orange o Cells stained with DAPI fluoresce bright blue, whereas cells stained with acridine orange stain orange or greenish-orange o DAPI and acridine orange staining are widely used for the enumeration of microorganisms in environmental, food, and clinical samples o Staining with either DAPI or acridine orange is nonspecific; all microorganisms in a sample will stain Viability Staining o Viability staining allows one to differentiate live cells from dead cells
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 03/06/2012 for the course BIMM 120 taught by Professor Larsen during the Spring '08 term at UCSD.

Page1 / 2

BIMM 120 Lecture16_ReadingGuide.052411 - Lecture 16...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online