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Topic 2 - • If exponential phase cells are added to a...

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Topic 2 2.1 Cell Growth versus Population Growth Most bacterial cells reproduce by binary fusion Cell cycle of bacterial cell: o Elongation o Chrosome segregation o Septum formation o Cell division 2.2 Bacterial cell Population growth Lag Phase : cells adapt to a medium, cells synthesize new proteins that are needed for growth, if cells added were growing in the same conditions previously might not be lag phase. Exponential phase : Cells grow exponentially as the medium allows Station Phase : The loss of cells due to death is balanced by increase due to division, cell number stops increasing Death Phase : number of cells begins to decrease, cell death rate increases, cells adapt to very slow growth and use up nutrients slowly, they adapt to living conditions where they are living off material released on death of other cells.
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Unformatted text preview: • If exponential phase cells are added to a death phase population, the exponential phase cells die rapidly because they have not undergone gradual metabolic adjustment • End point of a culture depends on the medium and type of cells • Optimum conditions are moderate to high tmp and lots of organic nutrients • Cells growing quickly reach a bigger size in cells growing slowly this is because the rate limiting factor in growth rate is the number of ribosomes per cell and to grow faster (make proteins in short time) cells habe more ribosomes so that means they need to be bigger to accommodate their “machinery” • 10^6 and 10^9 cells per mL • Cells number increase due to binary fusion • N = N o 2 n or time/time takes to double •...
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