Slides 4 012411(2)

Slides 4 012411(2) - Today: 1) Using amino acid sequence to...

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Unformatted text preview: Today: 1) Using amino acid sequence to explore protein function I have posted answers to e-mails on Blackboard under Questions The PyMol program, a tutorial, and the Builds that I showed in class are in the Course materials section on Blackboard 2) Protein secondary structure 3) Protein domains 4) Multipeptide proteins: globular and fibrous 5) Protein binding: The globins Note: reading for the end of this lecture and some of the next is pp. In Lehninger, Principles of Biochemistry, 5th ed. pdf will be posted on Bb!! Summary: Protein structure Protein rearrangements are likely to have played a major role in human evolution Proteins have flexible structures and regions; this flexibility contributes to function Symmetry and regularity in structure often arises from repeated interactions of similar or identical subunits Proteins are folded into shapes that are defined by the regions of secondary structure and how they are linked together Similar types of interactions and bonding defines peptide Summary: Protein structure Protein rearrangements are likely to have played a major role in human evolution Proteins have flexible structures and regions; this flexibility contributes to function Symmetry and regularity in structure often arises from repeated interactions of similar or identical subunits Proteins are folded into shapes that are defined by the regions of secondary structure and how they are linked together Similar types of interactions and bonding defines peptide chain interactions at multiple levels Using primary structure (amino acid sequence) DNA sequencing projects allow prediction of protein sequences Results 1: protein alignments Results 2: protein motifs ca. 30% identity over protein- may be related by common ancestry Gene families- within an organism= paralogs between organisms= orthologs Short regions of alignment: often have a specific shared function Using primary structure (amino acid sequence) BLAST at NCBI using E. coli adenylate kinase identifies Human ortholog 54% identity Identity over length of polypeptides Evolution by gene duplication and divergence Results 1: protein alignments Voet and Voet. pp. 192-194 Planorbis Lamprey Shark Platypus Human Monkey Model for Evolution of Globins (Geis) Using primary structure (amino acid sequence)...
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Slides 4 012411(2) - Today: 1) Using amino acid sequence to...

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