101811 ER-Golgi

101811 ER-Golgi - PROTEIN SYNTHESIS IN ENDOPLASMIC...

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PROTEIN SYNTHESIS IN ENDOPLASMIC RETICULUM
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INTRACELLULAR MEMBRANE ORGANELLES
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George Palade The Pancreatic Exocrine Cell UNDERSTANDING THE PROTEIN TRAFFICKING 1974 The Nobel Prize in Physiology or Medicine Albert Claude Christian de Duve George E. Palade
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time R a d i o c t v e p r n Add 3 H amino acids Remove 3 H and add “cold” amino acids pulse chase PULSE-CHASE EXPERIMENTS
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Label in RER Label in Golgi Label in condensing granules Label in dense granules RESULTS OF 3 MIN PULSE FOLLOWED BY CHASE IN PANCREATIC EXOCRINE CELLS
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0 25 50 75 100 % of total grains lying over 0 20 40 60 80 100 120 time (minutes) RER Golgi condensing granules zymogen granules Analysis of grain distribution after Pulse Chase Labeling of Exocrine Cells
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SECRETORY PATHWAY IN PANCREATIC CELLS
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TYPES OF ER Smooth ER : - Lipid synthesis (esp. hepatocytes) - Exit sites for newly synthesized proteins (all cells) - Calcium storage Rough ER : - Protein synthesis (transmembrane, luminal, and secreted proteins)
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MEMBRANE TRAFFICKING PATHWAYS IN THE CELL
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101811 ER-Golgi - PROTEIN SYNTHESIS IN ENDOPLASMIC...

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