T he inner m itochondrial m embrane has tw o related

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Unformatted text preview: p ts . T he inner m itochondrial m embrane has tw o related p hysical p roperties th a t m a k e it d iffe re n t fro m o th e r m e m b ra n e s in th e e u c a ry o tic c e ll, a n d su g g e st its e v o lu tio n a ry o rig in . W h a t a re th e s e two p ro p e rtie s ? L is t th e m in th e b o x e s b e lo w. 1 It is extrem ely protein rich – m em brane proteins com prise approxim ately 75% o f its content 2 It is extrem ely im perm eable to solutes (c ) 2 p ts . T he outer m itochondrial m embra ne als o has a n unusual p roperty, n ot comm on to other m embra nes in the eucaryotic cell. W hat is that p roperty, a nd w hat c la s s o f p ro te in s c a u s e s it? L is t th e m in th e b o x e s b e lo w. U n u su al p ro p erty o f O MM: It is very leaky to sm all s olutes (below 1 000 D a) M em b ra n e p ro tein responsible: PO R IN S, a c lass o f b eta-barrel m em branesp an n in g p ro tein s, fo rm n o n -s p ecific ch an n els in th e IMM (10) You are working with mitochondria in cells under the following conditions: The cytoplasmic -12- NAME: __________KEY________________ MIDTERM EXAM II BIOLOGY 231 19 October 2009 ________________________________________________________________________________ pH = 7, the matrix pH = 8, the potential across the IMM (Ø) = 100 mV, and under matrix conditions the synthesis of ATP from ADP and Pi has ÄG = 60 kJ/mo...
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This note was uploaded on 03/12/2012 for the course BIO 231 taught by Professor Hollenbeck during the Fall '11 term at Purdue University-West Lafayette.

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