A 6 pts calculate the g of the h gradient across the

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Unformatted text preview: le. (a) 6 pts. Calculate the ÄG of the H+ gradient across the IMM. Show your work and WRITE your final answer in the BLANK at LOWER RIGHT. Be careful with UNITS. F o r a n IM M p ro to n g ra d ien t, Ä G = F (Ä p), w here Ä p = ø ￿ (59m V ￿ Ä p H ), ø = V m o f the IM M , a nd Ä pH = (pH cytoplasm /IMS ￿ p H m atrix) H ere, Ä pH = 7￿ 8 = ￿ 1 S o Ä p = 1 0 0 m V ￿ (59 m V × ￿ 1 ) = 1 5 9 m V Ä G H = F (Ä p ) = 0 .1 kJ/mV ￿ m o l × 1 5 9 m V = 1 5.9 kJ/m ol F IN A L A N SW ER :__ 15.9 kJ/m ol__ (b ) 2 p ts . W hat is the en ergetically favorable direction o f flow f or H + u n der these conditions ? IN TO the m atrix (out o f the cytoplasm /IM S; s ince the Ä G H > 0) ( c) 4 pts. What is the minimum number of protons that will have to flow through the ATP synthase in order to synthesize one molecule of ATP from ADP and Pi under these conditions? Answer with an INTEGER. Very briefly, give your reasoning. num ber F OUR r ea soning 4 m oles H + × 1 5.9 kJ/m ol = 6 4 kJ; just enough to m ake o ne m ole o f A TP (60 kJ), if the synthase c om plex is 9 4% efficient -13- NAME: __________KEY________________ MIDTERM EXAM II BIOLOGY 231 19 October 2009 ________________________________________________________________________________ (EC) In honor of Elizabeth Blackburn, Carol Greider, and Jack Szostak recently winning the 2009 Nobel Prize for Medicine for their discovery of and work on telomeras...
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This note was uploaded on 03/12/2012 for the course BIO 231 taught by Professor Hollenbeck during the Fall '11 term at Purdue University-West Lafayette.

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