Second reading F Ch 19, next gen handout

Second reading F Ch 19, next gen handout - Emily Lin...

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Emily Lin 1/25/2011 F 19.1 - 19.4, handout - Next Gen Sequencing Pre-read This chapter discusses how genetic recombinant DNA technology has been utilized to improve our understanding of how genes work and advance biotechnology. In section 19.1, it overviews the effort made to cure pituitary dwarfism in humans, and caused by defective copies of GH1 and an autosomal recessive trait. When GH1 isolated from cadavers was used as a while to treat victim it caused prion protein infections; thus, researchers inserted GH1 into E. coli to grow large quantities of recombinant bacterial cells that would produce uncontaminated growth hormone sufficiently. Reverse transcriptase catalyzes the synthesis of DNA from RNA, and this is called complementary DNA (cDNA). Usually, researchers add a primer to single stranded cDNA and use DNA polymerase to complete. They used mRNA from the pituitary gland and reverse transcribed these into cDNA, which were cloned. Cloning was enabled by inserting it into a plasmid, called a vector, which is placed in a bacterial cell. They also used restriction
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Second reading F Ch 19, next gen handout - Emily Lin...

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