third reading freeman ch 20, klug 14.5

third reading freeman ch 20, klug 14.5 - Emily Lin 2/1/11...

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Emily Lin 2/1/11 Freeman Chapter 20, Klug 14.5 Genomics is the effort to sequence, interpret and compare whole genomes. Shot-gun sequencing is when a sequence is broken up into a set of overlapping fragments that are small enough to be sequenced. Each piece is then inserted into a plasmid called a bacterial artificial chromosome (BAC). Then DNA is cut again into 1 kb fragments into plasmids and replicate. Then the sequenced fragments are put back into the correct order. Good coding sequences are indicated with the computer that highlights long stretches of codons that lack a stop codon but are flanked by stop and start codon. The computer might also look for sequences typical of promoters, operators or other regulatory sites. DNA segments that are identified using this method are called open reading frames, or ORFs. Homologous genes often point to similar ancestry and also have similar base pair sequences/ function. Thus ORFs can be confirmed as genes by using homology. It’s difficult to sequence data in eukaryotes because coding regions are broken up by introns and because majority of DNA doesn’t code for anything. Several observations can be made in bacterial and archaeal genomes thus far. In bacteria,
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This note was uploaded on 03/04/2012 for the course BIO 142 taught by Professor Escabar during the Spring '08 term at Emory.

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third reading freeman ch 20, klug 14.5 - Emily Lin 2/1/11...

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