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Unformatted text preview: 14.3 To transfer a gene from one organism to another: 1) a source of DNA that contains the gene you want to transfer 2) a restriction enzyme to cut the SNA 3) a vehicle to carry the source DNA into the host cell DNA Library- a collection of DNA fragments representing all fo the DNA from an organism. Vector =vehicle a molecule of DNA that can carry the gene into a cell. Eg: bacterial plasmids and viruses. 4 stages of gene transfer 1) cleaving DNA- cutting the source and vector DNA 2) Producing recombinant DNA-placing the DNA fragments into vectors that transfer DNA into the target cells 3) Cloning-introducing DNA-bearing vectors into target cells and allowing the cells to reproduce 4) Screening-selecting the infected cells that have received the gene of interest. Restriction enzyme is used to cleave the source DNA into fragments. Electrophoresis- separates fragments from each other according to their size....
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- Fall '11
- DNA, source DNA