05 - Growing cells in the laboratory 1. To study cell...

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Unformatted text preview: Growing cells in the laboratory 1. To study cell physiology 2. To synthesize valuable cell product Gene cloning + Æ Recombinant protein Cell culture e.g. vaccines, monoclonal antibody, recombinant glycoprotein With HGF Without HGF Culture medium (i) Glucose (ii) 13 Essential amino acids (iii) Vitamins (iv) Salts (v) Antibiotics (vi) Serum (e.g. FBS) Arginine 0.1 Biotin 10-3 NaCl 100 Cysteine 0.05 Choline 10-3 KCl 5 Glutamine 2 Folic acid 10-3 NaH 2 PO 4 ·H 2 1 Histidine 0.05 Nicotinamide 10-3 NaHCO 3 20 Isoleucine 0.2 Pantothenic acid 10-3 CaCl 2 1 Leucine 0.2 Pyridoxal 10-3 MgCl 2 0.5 Lysine 0.2 Thiamine 10-3 Methionine 0.05 Riboflavin 10-4 Phenylalanine 0.1 Threonine 0.2 Tryptophan 0.02 Tyrosine 0.1 Valine 0.2 Miscellaneous Glucose Penicillin* Streptomycin* Dialyzed horse serum 5% *To prevent microbial contamination Source: Science 1955 122:501 Salts (mM) 5 mM 0.005% 0.005% L-Amino Acid (mM) Vitamins (mM) Cell dissociation and separation techniques Tissue cultures Tissue Dissociation...
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05 - Growing cells in the laboratory 1. To study cell...

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