Biol140L_Microorganisms and Environments They Grow in

Biol140L_Microorganisms and Environments They Grow in -...

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Biol 140L Lab Report – Ta Page 1 Microorganisms and Environments They Grow In Biol 140L Lab Report Experiment #20 and 21 Performed On: June 23, 2011 - June 30, 2011 Vyvy Ta - 20229611 Lab Partner: Aisling Finan - 20264998 Teaching Assistant: Allison Felker and Silvija Mihajkovic Section 003 – Thursdays at 2:30PM
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Biol 140L Lab Report – Ta Page 2 Introduction: This report focuses on microorganisms and the environmental conditions that are best for them to live and reproduce in. The experiment used Staphylococcus aureus, Serratia marcascens, Pseudomonas fluorescens, Bacillus stearothemophilus, Saccharomyces cerevisiae, Aspergillus niger, Escherichia coli, and Lactobacillus plantarum as bacteria to test on. These specific species were used because they all grow in different environments in the wild. The metabolism of bacteria can be proportional to the temperature that it lives in. The best temperature is one that helps their biochemical reactions to be more efficient (Madigan et al., 2011). Bacteria does not grow at one temperature, it can grow between a minute to wide range. Within that range is an optimal growth temperature at which they are the most productive and efficient at (Madigan et al., 2011). If the temperature is too low for a microorganism, growth is not possible (Madigan et al., 2011). That is because the low temperature slows down the metabolic reactions occurring inside of the organism (Madigan et al., 2011). The optimum temperature for an organism is close to the maximum temperature that it will survive in (Madigan et al., 2011). A microorganism will not grow at its maximum temperature or past it because the high temperature can cause the enzymes and proteins in it to denature (Madigan et al., 2011). The pH of a microbial organism’s environment affects their growth and survival as well. Microbial organisms carry out their functions using enzymes (Madigan et al., 2011). The pH affects these functions because of the hydrogen ions that are associated with pH (Madigan et al., 2011). Like with temperature, there is also an optimal pH at which a microbial organism can function at (Madigan et al., 2011). Microorganisms all have different specifications for optimal temperature and pH. They are all not the same because they may have different cell wall structures and the enzymes that they produce to survive are different as well (Madigan et al., 2011). With our knowledge in temperature and pH, we are able to combat against microbial growth to create sterile environments. Many tools for medical use are sterilized by using hot temperatures to kill microorganisms. There are foods that we eat that are a certain pH to prevent
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Biol 140L Lab Report – Ta Page 3 microbial growth as well. Manufacturers will add acidic chemicals to preserve and sterilize food products (Madigan et al., 2011). The objective of this report is to determine what environment is optimal for microorganisms to grow at, but also to observe when they will cease to grow. Materials and Methods:
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This note was uploaded on 03/12/2012 for the course BIOL 140L taught by Professor Duxbury during the Spring '11 term at Waterloo.

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Biol140L_Microorganisms and Environments They Grow in -...

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