AT Lecture 14

AT Lecture 14 - BISC 330L: Biochemistry Spring Semester...

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Unformatted text preview: BISC 330L: Biochemistry Spring Semester 2012 USC Lecture 14: Friday 10 February 2012 1. Chapter 8 Enzymes: Basic Concepts and Kinetics Free energy and enzymes ES complex Active sites Michaelis-Menten Kinetics Current events in biochemistry G and enzymes A + B C + D G = G o + RTln [C][D]/[A][B] 0 = G o + RTln [C][D]/[A][B] ( At Equil, G=0 ) G o = -RTln [C][D]/[A][B] Equil constant, K eq = [C][D]/[A][B] G o = -RTln K eq o R = 8.315 x 10 -3 kJ/mol deg T = 298 K (25 o C) Example: DHAP GAP G o = -2.303RTlog 10 K eq G o = -2.303 x 8.315 x 10-3 x 298 x log 10 (0.0475) G o = + 7.53 kJ/mol (+1.80 kcal/mol) Assume DHAP = 2x10-4 M and GAP = 3x10-6 M G = + 7.53 kJ/mol + 2.303RTlog 10 (3x10-6 M)/ (2x10-4 M) G = + 7.53 kJ/mol 10.42 kJ/mol G = - 2.89 kJ/mol (-0.69 kcal/mol) Enzymes alter reaction rate and NOT the reaction equilibrium S P 10-4 s-1 10-6 s-1 K = [P]/[S] K = k F /k R K = 10-4 s-1 /10-6 s-1 K = 100 Enzymes lower Activation Energy Activation Energy (E A...
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AT Lecture 14 - BISC 330L: Biochemistry Spring Semester...

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