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AT Lecture 15 - BISC 330L Biochemistry Spring Semester 2012...

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BISC 330L: Biochemistry Spring Semester 2012 USC Lecture 15: Monday 13 February 2012 1. Chapter 8 – Enzymes: Basic Concepts and Kinetics K M and V max k cat /K M Three types of inhibitors
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K M and V max K M is [S] at V max /2 K M is a measure of the strength of ES High K M = Weak Low K M = Strong • V max is turnover number V max = k 2 [E] T
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What is k cat /K M ? V o = k cat /K M [S][E] (14)+(20) • [S]<<K M so V o = k cat /K M [S][E] T k cat /K M is a measure of catalytic efficiency k cat is rate of catalysis K M is strength of E-S interaction Catalytically perfect enzymes
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Catalytically perfect enzymes: k cat /K M between 10 8 - 10 9 s -1 M -1
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Types of inhibitors Irreversible Reversible Competitive Uncompetitive Noncompetitive http://alexandria.healthlibrary.ca/documents/notes/bom/unit_1/L-20%20Enzymes%201%20and%202.xml
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The ES complex
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Competitive inhibitor
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Competitive Inhibition Specific inhibitor binds to active site and prevents substrates from binding
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Uncompetitive inhibitor
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Noncompetitive inhibitor
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