C10-pk_width - Chem 227F 2010 C10: Peak Width Analysis Due...

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Chem 227–F 2010 C10: Peak Width Analysis Due Thursday, November 18 Objective: Learn to use a spreadsheet to determine the full width at half maximum (w 1/2 ), number of theoretical plates (N) and resolution of peaks in a separation. Then be able to compare N and the resolution between two closely spaced peaks in a pair of separations of the same mixture performed under different conditions. The “efficiency” of a chromatographic separation is described by the number of theoretical plates. The equations for calculating N and resolution between chromatographic peaks are also applied to other kinds of chemical separations, including capillary electrophoresis (CE). For this assignment, you will use two CE runs from seminal work. In the early to mid 1990s, considerable interest emerged in the miniaturization of chemical analysis because of the potential for rapid analysis times and reduced reagent consumption. Perhaps the greatest success in miniaturization has been achieved with microfabricated CE devices; in fact, several companies have now commercialed this technology. The files ce1.csv and ce2.csv contain some of the first DNA separations obtained in a microfabricated CE device. These separations (or “electropherograms”) of the X174 Hae III restriction digest, which were published in 1994, were as much as an order of magnitude faster than any previously reported DNA separations. Create a spreadsheet that will allow you to calculate w
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This note was uploaded on 03/15/2012 for the course CHEM 227 taught by Professor Stevengoates during the Fall '10 term at BYU.

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C10-pk_width - Chem 227F 2010 C10: Peak Width Analysis Due...

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