Exercise #2 pre-lab - Name_Wendy Mei 1(4 points When one...

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Name____Wendy Mei___________ 1. (4 points) When one does an enzyme kinetics experiment, two important parameters are determined. Briefly explain what is meant by Km and V max. Km is the substrate concentration at where the velocity is equal to a half of the Vmax, which measures the strength of binding of the substrate to the active site. If there is a high Km, there is weak binding and vice versa. Vmax is where the velocity reaches a maximum where the available active sites are filled and adding more substrate will not change the rate of the reaction past Vmax. 2. (2 points) In our experiment, we will be varying substrate concentrations in the mM range and we will determine changes in product at the level of nmoles per minute. With this information, indicate the correct units for Km and Vmax. mM- Km nmoles/min -Vmax 3. (4 points) How does a competitive inhibitor affect Km and Vmax? A non-competitive inhibitor? A competitive inhibitor should increase the Km, but not alter the Vmax if enough subrate is
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  • Spring '12
  • Tepperman
  • Enzyme inhibitor, vmax, enzyme kinetics experiment, mM- Km, standard enzyme dilution, true non-competitive inhibitor

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Exercise #2 pre-lab - Name_Wendy Mei 1(4 points When one...

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