Exercise #6 Pre-lab - Name_Wendy Mei_ 1. (1 point) In...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Name_____Wendy Mei_______ 1. (1 point) In passing electrons from succinate to FAD, the succinate is a. reduced b. oxidized 2. (1 point) If DCIP is blue in the oxidized form and colorless in the reduced form, will we be measuring the a. gain of color b. loss of color 3. ( 2 points) Why is the sodium azide used in this experiment? What do you predict will happen if it is omitted and why? Sodium azide is used in this experiment because it will inhibit the electron transport chain and will be pushed more towards the DCPIP and not the electron transport chain. If sodium azide is omitted from the experiment, the reaction will go onto the electron transport chain and will oxidize the FADH 2 produced from the preceding reaction and we will not be able to monitor the activity of succinate dehydrogenase. 4. (1 point) In the preparation of the broken mitochondria, what will happen to the mitochondrial DNA? The mitochondrial DNA will be placed into a lower pH solution to make the membranes leaky and then a pellet with be obtained through centrifugation. The pellet will be placed into a water
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 3

Exercise #6 Pre-lab - Name_Wendy Mei_ 1. (1 point) In...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online