Exercise #4 Pre-lab (week 2)

Exercise #4 Pre-lab (week 2) - Name:_Wendy Mei_ 1. (6...

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Name:_Wendy Mei_____________ 1. (6 points) SDS gels are used to determine the molecular weights of proteins, but non-denaturing gels are not. Explain the basis of separation in non-denaturing gels and denaturing (SDS) gels. Separation in non-denaturing gels: proteins are loaded to the top of the gel (subjected to an electric field), proteins move based on size, charge, and shape. It cannot be used to determine the molecular weights of proteins because the migration of proteins is affected by other factors. Separation in denaturing (SDS) gels: proteins are denatured by heat and the interactions with sulfhydryl reducing agents (β-mercaptoethanol, disrupts cysteine-cysteine disulfide bonds), and detergents (SDS). When the protein interacts with the SDS, the nonpolar tail interacts with the non-polar amino acids in the protein and the polar head group interacts with water, which increases the solubility of the protein and causing the protein to have a linear shape. When proteins are covered with SDS, it has a large negative charge on the
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Exercise #4 Pre-lab (week 2) - Name:_Wendy Mei_ 1. (6...

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