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Unformatted text preview: reaction you reach half‐life (8.95 Activity) between 7 and 8 minutes. However, for the 10 nM reaction, you do not reach this point until after 15 minutes (the half life is actually 23.8 minutes). This relationship between starting concentrations and half‐lives, increasing initial [E] decreases the half‐life, also fits with a 2 nd order process. One possible explanation for the observed inactivation and reactivation is that this protein is only active in its dimeric form. The enzyme is inactivated at 60 ° C due to the dimers dissociating into inactive monomers. As two monomers bind to form a dimer at 5 ° C, they regain their activity: 0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 0.18-5 5 10 15 20 Second Order Fit 1/[E], 10 nM 1/[E], 30 nM y = 0.10361 + 0.0042163x R= 0.99728 y = 0.033465 + 0.0041852x R= 0.99903 1/[E] Time (min)...
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This note was uploaded on 03/16/2012 for the course MCB MCB 123 taught by Professor Christopherfraser during the Spring '11 term at UC Davis.
- Spring '11