MCB121 W12 L1_L2 practice problems

MCB121 W12 L1_L2 practice problems - MCB121 practice...

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MCB121 practice problems I. DNA and Genomes I-1. Name one way that LINE and SINE elements are similar and one way they are different (other than one is long and the other is short). I-2. One gram of cultured human cells contains roughly 10 9 cells. The diploid human genome comprises 6.4 x 10 9 base pairs. If base pairs occur at an interval of 0.34 nm, how long is the total length of DNA in this sample in kilometers? I-3 The number of genes encoded by the human genome is similar to the number of genes in the nematode Caenorhabditis elegans (~20,000). The size of the human genome in base pairs, however, is about 300 times greater. List three types of DNA sequence elements that are overrepresented in the human genome to account for the differences in genome size between the two organisms. I-4. On the diagram below, label the adenine, guanine, thymine and cytosine structures. Indicate which bases are purines and which are pyrimidines
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I-5. Gillyweed is a magical plant that when eaten gives a person gills to breathe underwater and webbed hands and feet for swimming. Harry Potter used it for the underwater event at the tri- wizard tournament. Professor Sprout has assigned her herbology class to determine the genome sequence of this plant. A. Briefly outline a general strategy (in 4 or 5 steps) that you would use to create a draft sequence (about 4X coverage) of the Gillyweed genome without using any magic. B. You find that the Gillyweed genome has ~2400 Mb of DNA. Give a rough estimate of how many genes there are in Gillyweed genome? Explain your answer. Open-ended questions: I-6. Which of the following forms of DNA (double-stranded DNA, single-stranded DNA, and free nucleotides) would have the lowest ultraviolet light absorbance? Why? Assume that each is in solution at the same concentration.
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I-7. What is referred to as the melting temperature of DNA, and how is it influenced by the relative amount of G-C versus A-T base pairs? I-8. Discuss how the ionic strength of DNA in solution (at or near physiological conditions) affects the melting temperature (T m ).
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II. Chromosome structure II-1. For each set circle the chromatin type that would exhibit the highest packing ratio, i.e. most condensed (Circle one of each pair): 30 nm fiber / 10 nm fiber Euchromatin / Heterochromatin Metaphase chromosome / Interphase chromosome Nucleosome wrapped DNA / DNA loops attached to a scaffold II- 2. It is widely known that humans will become Zombies within several hours of experiencing a Zombie bite. The prevailing theory is that the human to Zombie state is caused by infection with HZV (Human Zombie Virus) transmitted through Zombie saliva. You have just been hired to work at the Institute for Zombie Studies to test the hypothesis that the HZV genome has integrated into the DNA of Zombie brain cells and test for other possible chromosome-based changes these cells. A.
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This note was uploaded on 03/16/2012 for the course MCB MCB 121 taught by Professor Gasser,burgess during the Winter '11 term at UC Davis.

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MCB121 W12 L1_L2 practice problems - MCB121 practice...

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