MCB121 MT2 GreenKey

MCB121 MT2 GreenKey - Name Green Key Last MCB121 Page 1 of...

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Name Green Key MCB121, Page 1 of 9 Last, First Gasser, Burgess ©2012 Charles S. Gasser, my not be reproduced without permission of author. Second Midterm, Feb 28, 2012 MCB121 Instructions: There are nine pages in this exam including the cover sheet, please count them before you start to make sure all are present. Write your name on each page of the exam. Write your answers in the space provided below each question. If you need more space use the back of the page and indicate clearly that you have continued your answer on the back. Do not use additional paper. Amino acid Abbreviations Amino acid Abbreviations Alanine Ala A Leucine Leu L Arginine Arg R Lysine Lys K Asparagine Asn N Methionine Met M Aspartic acid Asp D Phenylalanine Phe F Cysteine Cys C Proline Pro P Glutamine Gln Q Serine Ser S Glutamic Acid Glu E Threonine Thr T Glycine Gly G Tryptophan Trp W Histidine His H Tyrosine Tyr Y Isoleucine Ile I Valine Val V
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Name Green Key MCB121, Page 2 of 9 Last, First Gasser, Burgess ©2012 Charles S. Gasser, my not be reproduced without permission of author. Question Value Score 1 16 2 19 3 22 4 12 5 15 6 12 7 9 8 15 9 15 10 15 Total 150 1. A variety of methods were described in class for the isolation of specific sequences from libraries of clones. For each of the following, describe the kind of library (nature of insert and type of vector), and the nature of the probe you would use to isolate the desired clone. a) (8) You have a cDNA clone of the human Fos transcription factor, which includes a leucine zipper. You want to identify clones of genes encoding potential dimerization partners of Fos. Library to screen: xpression library of human mRNA from cells expected to be expressing Fos. Must be in an expression vector (plasmid or phage). (2-hybrid library if they happen to know about this is okay as well) Probe to use: Labeled Fos protein. b) (8) You have a cDNA clone of the rabbit ß-globin gene. You want to know what the next ten genes are in the mouse genome sequence downstream (3’ of) the ß-globin gene. Library to screen: Large insert genomic library of rabbit DNA, BAC vector preferred Probe to use: Labeled cDNA clone of the rabbit ß-globin gene
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Name Green Key MCB121, Page 3 of 9 Last, First Gasser, Burgess ©2012 Charles S. Gasser, my not be reproduced without permission of author. 2. (19) You have identified a new nuclear hormone receptor in mice that binds the novel hormone “XA”. You name it the XA receptor (XAR) because it is the only receptor in mouse cells that binds the XA hormone. You are studying the role of XA and XAR in regulating expression of genes using chromatin immunoprecipitation (ChIP) and analysis of the ChIP products using a mouse promoter sequence microarray. Samples of a mouse liver cell line were grown in the absence (“–” on the figure below) or presence (“+” on the figure below) of XA hormone. Chromatin was extracted, sheared and precipitated with either no antibody (mock-precipitated), with α -NCOR antibody specific for NCOR (a co-repressor protein known to recruit histone deacetylases (HDAC)), or with
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This note was uploaded on 03/16/2012 for the course MCB MCB 121 taught by Professor Gasser,burgess during the Winter '11 term at UC Davis.

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MCB121 MT2 GreenKey - Name Green Key Last MCB121 Page 1 of...

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