BIS 104 PQ 14 - effect What is the experimental evidence...

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BIS 104 PQ 14 The data presented in figures #1 and #2 provide evidence that tumor cells that are stably transfected with activatable (WT) a5b3 integrin genes are capable of generating tumors when injected subcutaneously into SCID mice, and that these tumors grow larger than tumors from S752P cells. It has also been established that WT tumor cells probably have this growth advantage because of their ability to stimulate angiogenesis, as seen by measuring CD31 and laminin expression in the tumor tissue. The experiments presented in figure #3 are designed to determine the molecular mechanisms for how integrin proteins influence tumor growth (establish cause and
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Unformatted text preview: effect). What is the experimental evidence for the follow three conclusions that the authors make based on the results shown in figure #3 a, b, and c? 1. When conditioned medium (CM) from WT and mutant cells are compared for the presence of a variety of growth factors, the main difference is that WT cells secrete much more VEGF-A than do mutant cells. 2. 4-week WT tumors contain more VEGF-A mRNA than do tumors from mutant cells. 3. 4-week WT tumors contain more VEGF-A protein than do tumors from mutant cells....
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