BIS 104 PQ 22 - cells stimulated by a growth factor begin...

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BIS 104 PQ 22 22. Mff (mitochondrial fusion factor) is a protein synthesized in the cytoplasm of mammalian cells and post-translationally transported to the outer membrane of mitochondria. Describe how you would design a pulse-chase experiment to determine whether or not cultured
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Unformatted text preview: cells stimulated by a growth factor begin to actively synthesize this particular protein and transport it to the mitochondria. Use 1.0 uM 14 C-leucine for the pulse and 100 uM 12 C-leucine for the chase. List steps in chronological order....
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