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Unformatted text preview: fluorescence microscopy, between WT and the constitutively active cell line expressing D723R integrins. b. What amino acids are exchanged at position 723 in the mutant? c. What were the results of this comparison? d. Do the results support the hypothesis? The effect of integrin clustering on VEGF expression was tested by treating WT cells with either soluble LM609 ligand (anti- 3 antibody) or with cross-linked LM609 ligand. e. How was VEGF expression measured? f. Why do you think that the cross-linked ligand stimulated VEGF expression, but the soluble form of the ligand did not?...
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- Fall '10