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Unformatted text preview: vitronectin (vn). 2. Cells were lysed and the lysate was immunoprecipitated (IP) with an antibody directed against 3. 3. The immnoprecipitate was subjected to SDS-PAGE followed by western blotting (WB). 4. The blotted proteins were probed with (a) anti-phospho-Shc ab, (b) anti-Shc ab, and (c) anti- 3 ab. The blots were developed to detect ab-ag complexes. Describe the RESULTS obtained for each of the IP / WB combinations: (a) (b) (c) What CONCLUSIONS can be drawn from these results?...
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This note was uploaded on 03/16/2012 for the course BIOLOGY BIS104 taught by Professor Bejjamin during the Fall '10 term at UC Davis.
- Fall '10