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BIS 104 PQ 26-1 - vitronectin(vn 2 Cells were lysed and the...

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BIS 104 PQ 26 26. The data in Figure 6a show results of experiments designed to search for an intracellular (cytoplasmic) mediator for the outside-to-inside signal pathway involving α 5 β 3 integrins in LNCaP cells. These experiments were designed with the prior knowledge that when blood platelets are activated by integrin-ligand binding, an isoform of the adaptor protein Shc is recruited to bind to the cytoplasmic domain of α 5 β 3. EXPERIMENT: 1. Both WT and SP LNCaP cell lines were grown as either a suspension (sus) or adherent to
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Unformatted text preview: vitronectin (vn). 2. Cells were lysed and the lysate was immunoprecipitated (IP) with an antibody directed against β 3. 3. The immnoprecipitate was subjected to SDS-PAGE followed by western blotting (WB). 4. The blotted proteins were probed with (a) anti-phospho-Shc ab, (b) anti-Shc ab, and (c) anti-β 3 ab. The blots were developed to detect ab-ag complexes. Describe the RESULTS obtained for each of the IP / WB combinations: (a) (b) (c) What CONCLUSIONS can be drawn from these results?...
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