BIS 104 PQ 29 - Vn what effect did this have on VEGF...

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BIS 104 PQ 29 29. In S. De et al., Figure 6 parts a and b show that WT LNCaP cells activated by Vn (i.e., integrins get cross-linked and undergo clustering) is sufficient to increase the recruitment of p66Shc to the cytoplasmic domain of a5b3. Answer the following questions regarding data shown in Figure 6 parts c and d. A. When WT cells were transfected with a dominant-negative p66Shc (Y313F) and grown on
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Unformatted text preview: Vn, what effect did this have on VEGF secretion? In other words, compare the concentration of VEGF found in conditioned media from: a) untransfected cells (controls) b) cells transfected with WT p66Shc c) cells transfected with dominant – negative p66Shc B. What technique was used to assay the secreted VEGF in the conditioned media?...
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This note was uploaded on 03/16/2012 for the course BIOLOGY BIS104 taught by Professor Bejjamin during the Fall '10 term at UC Davis.

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