MT2-Sp10-key-HPLC only - Name_ GSI _ Question 5) HPLC of...

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Name__________________ GSI ____________________ Question 5) HPLC of Food Dyes Synthetic food dyes are cheap and very colorful, however some are irritating to the eyes and throat and others may even cause cancer. The two HPLC methods below work well for the analysis of food dyes. Method Stationary Phase Mobile Phase 1 Silica-C18 Phosphate buffer (pH 7) in H 2 O, Triton-X (soap) 2 Silica-C18 Acetonitrile (CH 3 CN): Acetic Acid Buffer (pH 4) a) Of the two methods, which do you think is greener or more environmentally friendly? Briefly explain. Method 1 is greener than Method 2. Method 2 uses an organic solvent for the mobile phase. Acetonritile is toxic. Method 2 also has a more acidic buffer than Method 1. Method 1 only uses water and soap and pH 7 buffer. Examine the structures on the next page. The dye molecules are all ionic compounds with acid-base properties, similar to NaEDTA. Examine the chromatograms and resolution data with varying pH. Schematics of the molecules used in Method 1 are shown below. The soap in the mobile phase forms a micelle around the dye molecules.
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This note was uploaded on 03/16/2012 for the course MATH 1B taught by Professor Reshetiken during the Spring '08 term at University of California, Berkeley.

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MT2-Sp10-key-HPLC only - Name_ GSI _ Question 5) HPLC of...

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