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Unformatted text preview: Schematics of the molecules used in Method 2 are shown below. The soap in the mobile phase forms a micelle around the dye molecules. Stationary phase particles Dye in water at pH7 Dye-soap complex b) At low pH the separation is very poor but raising the pH to ~7 helps the compounds to stick to the mobile phase. Please explain in terms of the structures above. c) Examine the flow rate data. The compounds separate quickly at a flow rate of 2 mL/min. The experimenters chose 1 mL/min for all the experiments. Why is this flow rate best? SiO 2 Dye Dye-2 Name__________________ GSI ____________________ Page 8 of 8 Data for the separation of food dyes. Dye Molecules order of elution: (1-tartrazine, 2-brilliant blue, 3-sunset yellow)...
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- Spring '08