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25 BIS101001, Spring 2012--Genes and Gene Expression, R.L. Rodriguez 2012 Constructing Genetic Maps A genetic map is a means of displaying the number of genes, their order along the chromosome and their relative distances from each other. This is typically done using a test cross to an F1 individual carrying different alleles (heterozygous) for at least two genes or at two loci.
26 March 19, 2012 BIS101001, Spring 2012--Genes and Gene Expression, R.L. Rodriguez 2012 3 Approaches to Constructing Genetic Maps Approach 1. Determining gene order by performing multiple, two factor crosses using overlapping loci. Gene order will be revealed by consistent map distances. Approach 2. Perform a 3-factor test cross (F1 x tester) and determine genetic distances first. As above, gene order will be revealed by consistent map distances between the three loci. This approach can be performed without rare double crossover progeny. Approach 3. Perform a 3-factor cross (F1 x tester) and determine the gene order first. This i...
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