Practice_Problem_Set3_Solutions

Practice_Problem_Set3_Solutions - 1. Facioscapulohumeral...

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1. Facioscapulohumeral muscular dystrophy type 1 (FSHD1) is an autosomal dominant disorder tightly associated with the deletion of tandem repeats, each 3.3kb in length, from chromosome 4. Unaffected individuals have 11 to 150 tandem repeats while individuals with FSHD1 have only 1 to 10 tandem repeats. Because the entire variable number tandem repeat (VNTR) region resides between EcoRI restriction endonuclease sites (see below), restriction fragment length polymorph isms (RFLPs) can be created upon EcoRI digestion. The RFLPs can be detected by Southern blot analysis using a radiolabeled probe complementary to a sequence on the restriction fragment proximal to the VNTR region. Part of Chromosome 4 EcoRI I : 1.5kb 2kb probe 3.3kb f I I lkb 1.5kb: n I tandem repeat number (n) varies Six individuals submitted their DNA for genetic testing to determine their risk for FSHD1. Shown below are the results of Southern blot analysiS. 330kb - - 168kb _ - 85.5kb -- 32.7kb - 19.5kb - 6.3kb _ Individual # 1 #2 #3 #4 #5 #6 2
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Which individual(s} will get the disease? Circle the correct answer(s}. A.#l @ @ @ E.#5 F.#6 Which individual(s} will not get the disease? Circle the correct answer(s}. @ B.#2 C. #3 0.#4 Individual 1 claims that two of these individuals are his parents. Which pair of individuals are most likely his parents? Circle the correct answer. A .. #2 and #3 B. #3 and #5 cf#2and#!) O. #4 and #5 E. #3 and #6 3
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2. Which statement(s) is/are true about RNA interference. Circle the correct answer(s). A. It is likely that miRNAs inhibit (after dicer processing) the mRNAs of the genes they are made from. B. miRNA processing only occurs in the nucleus. C. miRNA processing only occurs in the cytoplasm. D. The endonuclease activity of "Dicer" converts ds-shRNA into 21-23 bp RNA (siRNA) making it competent for interference. E. siRNA is not infectious. F. The helicase activity of RISC unwinds the template mRNA from the antisense-RNA of the siRNA. G. If miRNA and its target mRNA contain perfectly complementary sequences, miRISC cleaves the mRNA. H.
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Practice_Problem_Set3_Solutions - 1. Facioscapulohumeral...

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