Six - Protein Misfold

Six - Protein Misfold - What causes a protein to misfold...

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2/3/12 MCB 2000 Lecture 6 Part 2 What causes a protein to misfold? Shape = Destiny In Vivo vs. In Vitro
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Protein Denaturation: In Vitro Loss of secondary and higher order structure Proteins have optimal pH and temperature at which they function. Noncovalent interactions are disrupted; peptide bonds are not. 2/3/12 MCB 2000 Lecture 6 Part 2
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2/3/12 MCB 2000 Lecture 6 Part 2 How do proteins fold? What factors determine folding? The Classic Experiment: Folding of Ribonuclease Disulfide bonds Disulfide bonds
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2/3/12 MCB 2000 Lecture 6 Part 2 Ribonulcease Experiment & Disulfide Bonds Disulfide bonds Disulfide bonds
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2/3/12 MCB 2000 Lecture 6 Part 2 The Ribonuclease (RNase) Experiment I Has activity: degrades RNA Question: What happens to RNase activity?
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MCB 2000 Lecture 6 Part 2 The Ribonuclease (RNase) Experiment II 1. Dialyze out urea and -Mercaptoethanol 2. 3. Questions: 1. What happens to the structure of RNase? 2.
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Six - Protein Misfold - What causes a protein to misfold...

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