Six - What causes a protein to misfold

Six - What causes a protein to misfold - What causes a...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
What causes a protein to misfold? Shape = Destiny Protein Denaturation: loss of secondary and higher order structure proteins have optimal pH and temperature at which they function. noncovalent interactions are disrupted: peptide bonds are not. Protein denaturation means you will lose all higher order structure from secondary and up. However, primary structures will remain. Destroys all of the non covalent interactions. Detergent act primary to alter or disrupt hydrophobic interactions Urea break apart hydrogen bonds and ionic interactions Is irreversible and not the same as misfolding Misfolding refers to a folded conformation but just not the right one. Unfolding results in exposure of hydrophobic or sticky patches. How do proteins fold? What factors determine folding? folding can bring together amino acids that are far apart in the chain folding can create binding sites The classic experiment: folding of ribonuclease Ribonuclease 4 disulfide bonds(made by and oxidative process) enzyme - degrade RNA about 124 peptides long does not have a lot of hydrophobic residue The ribonuclease(RNase) experiment I
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 03/19/2012 for the course BIO 2000 taught by Professor Ewr during the Spring '12 term at Alabama.

Page1 / 3

Six - What causes a protein to misfold - What causes a...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online