Ap bio lab 9. - 1 Determining the rates of transpiration in...

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1 Determining the rates of transpiration in a basil and leaf plant when exposed to an ambient, humid, intense light, and windy conditions Advanced Placement Biology Mr. Brothwell Samuel Castrejon
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2 Abstract The use of a potometer that contained tubing with a calibrated pipette that read in milliliters was used to determine the effects of transpiration on a pepper plant and a basil plant when introduced to various environmental conditions. The conditions were for different conditions and they were as following: intense light, wind, humidity, and ambient. After a thirty minute time span the completion of the experiment as complete and the experiment had many patterns that were found in each condition. it was found that an ambient condition had no effect on the rate of transpiration in either the pepper plant and the basil plant though. The wind condition was found that it didn’t cause a great amount of effect on the rate of transpiration which may have been caused to many errors during the experiment. Humidity was found to cause the most water loss and the intense light condition causing the most water lost. This laboratory significant because it shows how different plants are affected by different environments. It also shows why certain specific plants strive in different environments and those that would suffer when placed in the same environment, this is significant in explaining why a cactus can live in an intense light environment because of its unique rate of transpiration. Materials and Methods The pipette that is calibrated to measure liquid substances in millimeters and tubing of a potometer were submerged in a shallow tray of a couple of inches water. It is important that enough water is drawn to eliminate bubbles within the tubing. The plant stem was cut while submerged to prevent air bubbles from being introduced to the xylem. The freshly cut stem was inserted into the open end of the tubing while both were submerged in water. The potometer completed by taping the tubing in between two table
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3 faucets in a “U” shape. It is important that no bubbles are found beneath the cut steam. If needed, seal with parafilm to prevent any bubbles under the cut stem. The end result of the potometer should resemble this labeled diagram. The potometer equilibrated for ten minutes before any readings were done. The plant, which was either Pepper or Basil was exposed to one of the four following conditions: humidity, intense light, ambient, or wind. Each plant was exposed to each condition. The leaves of each plant in different potometers were covered by plastic bags that had been sprayed with water to replicate a humid condition. A fishbowl containing water was placed in front of the leaves of the potometers that contained pepper or basil l and a light source to replicate an intense light condition. The potometers containing basil or pepper was placed in a room at room temperature to replicate an ambient condition. To replicate a wind condition, a fan was placed facing the potometers leaves that were either basil or
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Ap bio lab 9. - 1 Determining the rates of transpiration in...

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