lab3. evolution&natural selection-wrap up

lab3. evolution&natural selection-wrap up - "$...

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!"#"$! $ + Lab #3 Evolution, Natural Selection Wrap-up BIOL 1209 Fall 2011 + Today’s lab ! Quiz ! Replica plating review & data collection ! Morphs assignment ! Rat exercise ! Wrap-up discussion ! Writing Assignment 1
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!"#"$! ! + Replica plating- review ! Why did we use Replica Plating and not streaking? ! Number of colonies ! Colony LOCATION ! What does this tell you? ! What are the two possible 2 main possible hypotheses concerning how bacteria will respond to antibiotics? + Replica plating- review ! Natural selection ! Alt Hyp: Some colonies will be immune to antibiotics and survive. That survival will lead to selection of resistant bacteria. ! Induced mutations ! Alt Hyp: Some colonies will have a induced mutation in response to antibiotics. No observable pattern of survival of colonies Null Hyp: No difference in number of colonies
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!"#"$! & + Replica plating- predictions Stock Plate [High] Plate [Medium] Plate [Low] Plate Control Plate Stock Plate [High] Plate [Medium] Plate [Low] Plate Control Plate Selection : Will see a reduced number of colonies as we increase antibiotics (predictable change) Induced May see loss but it will be unpredictable among colonies Bacterial colonies adapt to the antibiotic and become resistant to them Naturally occurring differences in each colony predicts whether a colony will survive or not in the presence of antibiotic If a colony survives in the high plate it must be also present in the lower concentration plates + Replica plating- data collection ! Count colonies on plates or use the percent cover procedure (lab manual) ! Count colonies and look at LOCATION !
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