Pop_Ecology_Procedures

Pop_Ecology_Procedures - Intraspecies Competition 1. 2. 3....

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Intraspecies Competition 1. Collect NINE sterile culture tubes. 2. Label all the tubes using a permanent marker in the top one third of the tube. All tubes should have your initials, section number and replicate information (one each: ctrl-1, ctrl-2, ctrl-3, low- 1, low-2, low-3, high-1, high-2, high-3) 3. Pipette 1 ml of Chlamydomonas culture into control tubes. Pipette 0.5 ml of Chlamydomonas culture into low tubes. Pipette 3 ml of Chlamydomonas culture into high tubes. Use 1 ml pipetman with blue pipet tips. You will have to pipet 3 times for 3 mls. 4. Pipette 4 ml of Min (minimal media, a mixture of nitrates, phosphates and trace metals) into control tubes. Pipette 4.5 ml of Min into low tubes. Add 2 ml of Min to high tubes. Use 10 ml pipet with the green pipette-aid. 5. Seal all tubes with parafilm squares. 6. Invert the tubes several times to mix the contents thoroughly. 7. Measure the initial absorbance at 750 nm for all tubes. MIX THE CONTENTS OF EACH TUBE IMMEDIATELY BEFORE PLACING IT IN THE SPECTROPHOTOMETER. 8. Recheck blank in the spectrophotometer to assure machine is still blanked correctly. 9. Remove parafilm from sample tubes. 10. Place all the tubes in rack to be placed under fluorescent lights at room temperature. Recover rack loosely with the saran wrap provided. Record rack and space number. 11. Calculate the density of Chlamydomonas in the tubes using the standard curve (available in moodle). 12. Copy this procedure into your lab.
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Interspecies Competition Chlamydomonas in the presence of blue-green algae (choose one) Blue-green algae are bacteria, specifically cyanobacteria. 1. Copy this procedure into your lab. 2. Collect NINE sterile culture tubes. 3. Label all the tubes using a permanent marker in the top one third of the tube. All tubes should have your initials, section number and replicate information (one each: Ch-1, Ch-2, Ch-3, both-1, both-2, both-3, BGA-1, BGA-2, BGA-3) 4. Pipette 1 ml of Chlamydomonas culture into Chlamydomonas tubes and both tubes. Pipette 1 ml of blue-green algae culture (either Oscillatoria or Anabaena ) into blue-green algae tubes and both tubes. Use 1 ml pipetman with blue pipet tips. 5. Pipette 4 ml of Min (minimal media, a mixture of nitrates, phosphates and trace metals) into Chlamydomonas and blue-green algae tubes. Pipette 3 ml of Min into “both” tubes. Use 10 ml pipet with the green pipette-aid. 6. Seal all tubes with parafilm squares. 7. Invert the tubes several times to mix the contents thoroughly. 8. Measure the initial absorbance at 750 nm for all tubes. This is to measure the Chlamydomonas levels. MIX THE CONTENTS OF EACH TUBE IMMEDIATELY BEFORE PLACING IT IN THE SPECTROPHOTOMETER. 9.
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This note was uploaded on 03/20/2012 for the course BIOL BIOL 1209 taught by Professor Gregg during the Spring '09 term at LSU.

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Pop_Ecology_Procedures - Intraspecies Competition 1. 2. 3....

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