Lab 4 Observing Cells

Lab 4 Observing Cells - Erika Blair BELS 311 LAB Shannon...

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Erika Blair BELS 311 LAB Shannon Ulrich February 20, 2012 Title: Observing Cells Questions: How does boiling yeast affect the active transport mechanisms of the cells? What are the observable characteristics of cells with a light microscope? Predictions: If the active transport proteins denature when boiled, then the Congo red stain will not enter the cells when added to boiled yeast cells in a hypotonic solution. By observation with a light microscope, the cell membrane, cell wall, and nucleus will be distinguishable. For Background Information, Hypothesis, Experimental Design, Materials, and Procedure, please see attached carbon copy sheets. Data Summary: All of the data recorded in this lab was in the format of qualitative observation and drawings. The following four drawings are of the data collected in guided activity A, where yeast cell samples were drawn when they were boiled and unstained, unboiled and unstained, boiled and stained, or unboiled and stained. Unboiled, No Stain (A) Boiled, No Stain (B) 400x 0.1mm 400x 0.1mm
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Unboiled, Congo Red Stain (C) Boiled, Congo Red Stain (D) 400x 0.1mm 400x 0.1 mm Estimated Cell Sizes for Guided Activity A: Sample Estimated Cell Size A 1.667 μm B 1.11 μm C 1.667 μm D 1.11 μm Data Statement Summary for Guided Activity A: The only cells that visibly contained red dye inside the cell membrane were the cells in sample C, the unboiled and stained yeast cells. For the unstained samples A and B, there was no visible red. For sample D, only the solution surrounding the cells was red. Inquiry Activity A was not completed due to complications with using the yeast cells. The data collected for the Guided Activity B is below, in the form of labeled qualitative drawings. Cheek Cells and Methylene Blue Stain (A)
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400x 0.1mm Elodea Leaf Cells (B) 400x 0.1 mm Pond Water Sample (C)
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400 400x 0.1 mm *Note: The sample I brought to class contained no visible microorganisms, and so I used a water sample from a Winegradsky column prepared by Dr. Judy Schoonmaker. Estimated Cell Sizes for Guided Activity B:
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Lab 4 Observing Cells - Erika Blair BELS 311 LAB Shannon...

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