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RESPIRAT11 - Jim Bidlack BIO 1304 GENERAL BOTANY Lecture 11...

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Jim Bidlack - BIO 1304 GENERAL BOTANY Lecture 11 - Respiration & secondary metabolism I. Overview A. Photosynthesis (energy & CO 2 fixation) - chloroplasts B. Respiratory & secondary metabolism 1. Glycolysis - cytoplasm 2. Krebs (Citric acid cycle) - mitochondrion 3. Electron transport & oxidative phosphorylation - mitochondrion II. Glycolysis [glucose and fructose from Calvin cycle (photosynthesis)] A. Glucose - glucose-6-p - fructose-6-p - fructose 1,6-dp - PGAL (=DHAP) - 1,3-dp-glycerate - 3-p- glycerate - 2-p-glycerate - phosphoenolpyruvate - pyruvate B. Initial step(s) - energy requiring (2 ATP) C. Subsequent steps 1. Two - substrate-level phosphorylations (4 ATP) 2. One - reduction of NAD to NADH (2 NADH) D. Yield - 2 ATP & 2 NADH E. Final product - 2 pyruvates III. Alternatives after glycolysis A. Lactic acid - 2 ATP (no NADH) ... intense muscle activity (little O 2 available) B. Ethanol - 2 ATP (no NADH) ... fermentation C. Krebs Cycle - (2 ATP) and oxidative phosphorylation (32 ATP) IV. Krebs Cycle - mitochondrial matrix A.
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