CHEM114B - Lecture 25

CHEM114B - Lecture 25 - Final exam Mar. 23, 3:00pm-5:59pm...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Final exam Mar. 23, 3:00pm-5:59pm Sit one seat apart Fill in the entire bubble Bring Student ID Fill in ID in scantron bubbles (replace A by 1) Write names on all sheets of exam No calculator
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 Lecture 25 Lipids and Steroids Biosynthesis (II)
Background image of page 2
3 Important concepts 1. Understand the regulation of cholesterol synthesis Understand regulation on HMG-CoA reductase Understand receptor mediated endocytosis Transport of cholesterol and TAC by lipoproteins LDL endocytosis 2. Understand the synthesis of bile acids and steroids Understand the mechanism of cytochrome P450
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
4 HMG-CoA Reductase Membrane protein of ER Responsive to cholesterol levels — increase in HGM-CoA reductase levels (200x) Transcriptional regulation Translational regulation Regulatory degradation Activity regulated by phosphorylation (AMPK) — dephosphorylated form more active
Background image of page 4
5 Direct Effect of Cholesterol: transcription HMG CoA- reductase and ~20 other cholesterol biosynthetic genes Sterol regulatory element binding protein (SREBP) SRECBP cleavage activating protein (SCAP) SCAP: sensor In small vesicles
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
6 HMG-CoA Reductase • Transcriptional regulation Translation of reductase mRNA: inhibited by Mevalonate derivatives
Background image of page 6
7 HMG-CoA Reductase Reductase degradation: Membrane domain of the reductase sense [sterol] => proteolysis
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
8 HMG-CoA Reductase • Transcriptional regulation Translation of reductase mRNA: inhibited by Mevalonate derivatives Reductase degradation: Membrane domain of the reductase sense [sterol] => proteolysis Ubiquitination under some conditions Activity switched off by phosphorylation (AMP-activated Kinase) — cholesterol synthesis stops at low [ATP] All four mechanisms are modulated by receptors that sense cholesterol in the blood.
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 03/23/2012 for the course CHEM 114B taught by Professor Wang during the Spring '09 term at UCSD.

Page1 / 35

CHEM114B - Lecture 25 - Final exam Mar. 23, 3:00pm-5:59pm...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online