LECTURE 3 PART 2 - product ii Binding to active site is an...

Unformatted text preview: product ii. Binding to active site is an energonic chemical reaction - Independent chemical reaction - This specific binning powers induced fit and strains bonds of substrates to increase order and decrease enti'opy iii. Enzyme operatfi through two steps One Enzyme binding Two. {:atalysis I. Enzyme's effect on chemical reactions A. Gibbs tree energy diagram A. Effecls 1. EI'IZ'HTIE lower M5“, and increase k {rate constant] 1. lncreaseofkbyalot a} Small decrease in energy ofactiyation? Magnitudes ofgreater reaction Hates i. Ex. Catalase -: 1 billion times larger reaction rate for the decomposition of hydrogen peroxide Increases the rate by same amount for forward and reverse reactions Rate in both directions still dependent on concentmtion of both substrate and product 1. Most reactions freely proceed in both direction a} Thus they are substi'a‘le—prochct concentration driyen yia Le Chatelier's principle 4. Enzyme does not affect position of equilibrium; :56“ dog not change; It:Ell does fit charge 55“!“ I. Triose Phosphate Isomerase - Reaction Mechanism [Acid base mtalysis] A. Not in tailback. E. Additional reading on TED MebCT] 1. I'm — Need to do proton and electron pushing arrows C. Triose phosphate isomerase is an enzyme that functions in glycolysis 1. glucose {6 carbon] is cut into two 3 carbon molecules 1. Dihyrioxyacetone phosphate [DHAP] into glyceraldehydeS-phosphate IGBPJ 2. TPI catalyzes DHAP .2 car 3. Extremely reversable in both directions E‘FM 'L‘ _‘ fibril-INF}: Cadill- ill; 12:"; "=9” as 33> ETHC if!" Fan: 15 ...
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