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Unformatted text preview: CHEM 350: Introduction to Biological Chemistry
Brian Lee, Ph.D.
Ofﬁce: Neckers 146G or 324
Hours: 9:30am to 10:30am or by appointment
Textbook (required, U.S. edition only)
Fundamentals of Biochemistry, 3rd Ed., Voet, Voet & Pratt.
Study Guide (recommended)
Student Companion to Fundamentals of Biochemistry, 3rd Ed.
Tuesday 6:30 to 7:30 pm in Neckers 218
Thursday 5:00 to 6:00 pm in Neckers 410 Announcements
Undergraduate Research Opportunities
Research for credit (such as CHEM 396 or CHEM 496)
Student worker ($8.00 per hour) (http://www.siu.edu/~fao/jobs/)
Undergraduate Assistantships (http://www.siu.edu/~fao/jobs/)
McNair Scholars Program (http://www.siu.edu/~mcnair)
REACH Awards Competition (http://www.siu.edu/~reach/) (due Jan 30th)
Undergraduate Scholarships (due Jan 31st)
Chemistry Majors see Department website
Science Majors see College of Science website
Summer Research Experiences for Undergraduates (REU)
Read Chapter 4 Amino Acids
Chapter 4 Problems
• First Midterm Exam, Monday February 6th
– Chapters 1 through 5 • Help Desk
Tuesday 6:30-7:30pm in Neckers 218
Thursday 5:00-6:00pm in Neckers 410 Recombinant DNA
to study isolated
genes or to control
expression of genes
carrier or vector
for cloned DNA
- DNA Sequencing
- Gene Expression
bacteriophage, baculovirus, artiﬁcial chromosomes (BAC, YAC) How do we select a successful clone?
Cloning site disrupts the lacZ gene in the plasmid.
LacZ is beta-galactosidase which normal cleaves lactose
On agar plates containing Xgal failed clones turn blue
Successful clones produce no LacZ and are white DNA Libraries can be stored in larger vectors:
bacteriophage, bacterial artiﬁcial chromosome (BAC)
or yeast artiﬁcial chromosome (YAC) vectors for
genomic sequencing. Finding a sequence of interest through in situ hydridization
Requires a known sequence to probe the library
Libraries can be genomic DNA or complementary DNA
cDNA library are reverse transcription copies of mRNA Polymerase Chain Reaction – isolate and amplify DNA
1. Denature 2. Anneal 3. Polymerize Second cycle:
are shorter than
the parent strands.
The new strands
begin and end at
the primer sequences.
(not shown here) At the end of the second
cycle, we have unit length
strands deﬁne by the
location of the 5’ and 3’
primers. Further cycles
will amplify these products. The PCR product can
then be cloned into a
vector for transfection
in another organism.
express proteins from
a foreign source. Site Directed Mutagenesis
to change genes
A synthetic primer
can be used to
The new sequence
can alter the amino
of the expressed
protein in transgenic cells. ...
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