jj - Routine Urinalysis Test Strip This assignment...

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Routine Urinalysis Test Strip This assignment addressed the following objectives that are part of the urinalysis portion of BLD 324: Objectives: For each chemical test on a standard 10 test urinalysis strip: discuss the principle of analysis, possible interferents, limitations of the assay, confirmatory tests (when appropriate). Complete the assignment on computer so you have a copy that you can easily modify and use for study. Submit by the due date in the course schedule as assigned . It will be returned to you at the final exam so you can use it during the exam…so what kind of questions do you think I will be asking???? This assignment is not graded right vs wrong because it is open book. Also, you can copy directly from the book as it is a study tool for you BUT you will actually learn more if you paraphrase or otherwise put the information into your own words/shorthand. Analyte Principle of detection: how does the strip detect this analyte? Normal values and lower limit of detection (just ballpark these as they vary with different manufacturers’ products) Interferents/false reactions both positive and negative. Explain what causes these. Specific Gravity by dipstick This test is based on the apparent pKa change of certain pretreated polyelectrolytes in relation to ionic concentration. In the presence of an indicator, colors range from deep blue- green in urine of low ionic concentration through green and yellow-green in urines of increasing ionic concentration. Random urines may vary in specific gravity from 1.001 -1.035. 8 Twenty-four hour urines from normal adults with normal diets and normal fluid intake will have a specific gravity of 1.016-1.022." In the book: Chemstrip: 1.000 to 1.030 Multistix: 1.000 to 1.030 Falsely Low: - Glucose and urea concentrations > 1g/dL - A pH greater or equal to 6.5; add 0.005 Falsely High: - Protein approximately equal to 100 to 500 mg/dL - Ketoacids such as lactic acid, ketones
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Analyte Principle of detection: how does the strip detect this analyte? Normal values and lower limit of detection (just ballpark these as they vary with different manufacturers’ products) Interferents/false reactions both positive and negative. Explain what causes these. pH The test is based on the double indicator principle that gives a broad range of colors covering the entire urinary pH range. Colors range from orange through yellow and green to blue. Both the normal and abnormal urinary pH range is from 5 to 9. No interferences known; unaffected by protein concentration. Protein This test is based on the protein-error- of-indicators principle. At a constant pH, the development of any green color is due to the presence of protein. Colors range from yellow for "Negative" through yellow-green and green to green-blue for "Positive" reactions. Normally no protein is detectable in
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jj - Routine Urinalysis Test Strip This assignment...

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