Introduction - Ula Kaminski Microbiology and Transformation...

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Ula Kaminski Microbiology and Transformation Section 4 March 29 th 2011 Introduction - Lets focus on the topic of transformation, a technique which copies foreign genes into a host cell, more commonly known as cloning (Urbance, 1). Bacteria are capable of transformation on their own, however in lab we manipulated the transformation of E. coli by using a plasmid called pGLO. Plasmids are round, extra-chromosomal pieces of DNA that can be found most bacteria and hold the ability to replicate or express DNA in a bacterial host (Averbeck, 1). The plasmid we treated E. coli with has a gene that is resistant to the antibiotic ampicillin. Our goal was to figure out the percentage of E.coli that actually picked up the plasmid. We first made the cells more permeable with CaCl 2 then heat shocked them (Froger, 1). Next we created a serial dilution of our pGLO cell suspension, which we then applied to spread plates to achieve countable, isolated colonies. Results
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Introduction - Ula Kaminski Microbiology and Transformation...

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