urinalysis_multistix_info_sheet - Multistix 10 SG...

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Multistix ® 10 SG Urinalysis Reagent Strips Tests for Glucose, Bilirubin, Ketone (Acetoacetic Acid), Specific Gravity, Blood, pH, Protein, Urobilinogen, Nitrite and Leukocytes in Urine. SUMMARY AND EXPLANATION/INTENDED USE: Bayer Reagent Strips for Urinalysis are firm plastic strips to which are affixed several separate reagent areas. Depending on the product being used, Bayer Reagent Strips provide tests for glucose, bilirubin, ketone (acetoacetic acid), specific gravity, blood, pH, protein, urobilinogen, nitrite, and leukocytes in urine. Please refer to the carton and bottle label for specific reagent areas on the product you are using . Test results may provide information regarding the status of carbohydrate metabolism, kidney and liver function, acid-base balance, and urinary tract infection. The reagent test areas on Bayer Reagent Strips are ready to use upon removal from the bottle and the entire reagent strip is disposable. The strips may be read visually, requiring no additional laboratory equipment for testing. Certain configurations of strips may also be read instrumentally, using the CLINITEK ® * family of Urine Chemistry Analyzers and the appropriate Program Module or Program Card. Contact your product representative for further information. The directions must be followed exactly. Accurate timing is essential to provide optimal results. The reagent strips must be kept in the bottle with the cap tightly closed to maintain reagent reactivity. To obtain optimal results, it is necessary to use FRESH, well-mixed , uncentrifuged urine. CHEMICAL PRINCIPLES OF THE PROCEDURE: Glucose: This test is based on a double sequential enzyme reaction. One enzyme, glucose oxidase, catalyzes the formation of gluconic acid and hydrogen peroxide from the oxidation of glucose. A second enzyme, peroxidase, catalyzes the reaction of hydrogen peroxide with a potassium iodide chromogen to oxidize the chromogen to colors ranging from green to brown. Bilirubin: This test is based on the coupling of bilirubin with diazotized dichioroaniline in a strongly acid medium. The color ranges through various shades of tan. Ketone: This test is based on the development of colors ranging from buff-pink, for a negative reading, to purple when acetoacetic acid reacts with nitroprusside. Specific Gravity: This test is based on the apparent pKa change of certain pretreated polyelectrolytes in relation to ionic concentration. In the presence of an indicator, colors range from deep blue-green in urine of low ionic concentration through green and yellow-green in urines of increasing ionic concentration. Blood: This test is based on the peroxidase-like activity of hemoglobin, which catalyzes the reaction of diisopropylbenzene dihydroperoxide and 3,3',5,5'-tetramethylbenzidine, The resulting color ranges from orange through green; very high levels of blood may cause the color development to continue to blue. pH:
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This note was uploaded on 03/27/2012 for the course BLD 324 taught by Professor Doig during the Spring '08 term at Michigan State University.

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urinalysis_multistix_info_sheet - Multistix 10 SG...

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