Lecture 9 Transcription

Lecture 9 Transcription - Biol141 Foundations in Modern...

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Biol141 Foundations in Modern Biology Cellular Biology and Genetics Lecture 9 – Wednesday, September 14 th , 2011 Transcription Reading: Freeman, Emory 2 nd Ed. Chapter 15 (pages 289-296)
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Learning objectives At the end of this lecture, you will be able to: 1) Determine the amino acid sequence encoded by either the template or the non-template (coding) strand of a given DNA sequence. 2) Contrast prokaryotic and eukaryotic transcription. 3) Explain the initation, elongation, and termination steps of prokaryotic transcription. 4) Describe the processing of eukaryotic mRNA to form mature mRNA.
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1. Transcription is RNA synthesis from DNA The template strand is read by the enzyme RNA polymerase (not pictured). The non-template (coding) strand is similar in sequence to the base sequence of the transcribed RNA.
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Transcription is RNA synthesis from DNA Note the RNA polymer is being synthesized in a 5’ to 3’ direction ! ! that is, the phosphate group on the 5’ Carbon of the incoming nucleotide is undergoing a condensation reaction with the OH group on the 3’ Carbon of the target nucleotide , forming phosphodiester bonds.
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This note was uploaded on 04/02/2012 for the course BIO 141 taught by Professor Dr.cafferty during the Fall '11 term at Emory.

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Lecture 9 Transcription - Biol141 Foundations in Modern...

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