Lecture 17 Prokaryotic gene regulation II

Lecture 17 Prokaryotic gene regulation II - Biol142...

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Biol142 Foundations in Modern Biology II Cellular Biology and Genetics Lecture 17 – Wednesday, February 29 th , 2012 Prokaryotic Gene Regulation II Reading: Freeman, Emory 2 nd Ed. Chapter 17 (pages 312 - 314)
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Learning objectives At the end of this lecture, you will be able to: 1) Contrast the use of negative and positive control of gene transcription. 2) Describe the roles of lactose and the repressor protein coded for by the lacI gene. 3) Explain the role of an operator sequence in a prokaryotic operon, using the lac operon as an example.
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E. coli genes involved in lactose metabolism were named lacZ , lacY , and lacI . ! lacI - mutants are called constitutive mutants because they produce ! -galactosidase and galactoside permease even when lactose is absent . lacZ - mutants lack functional ! -galactosidase ! lacY - mutants lack the membrane protein galactoside permease and so cannot transport lactose into the cell. What kind of protein might
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Lecture 17 Prokaryotic gene regulation II - Biol142...

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