Lecture 16

Lecture 16 - Biol142 Foundations in Modern Biology II...

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Biol142 Foundations in Modern Biology II Cellular Biology and Genetics Lecture 16 – Monday, February 27 th , 2012 Prokaryotic Gene Regulation I Reading: Freeman, Emory 2 nd Ed. Chapter 17 (pages 307 - 312)
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Learning objectives At the end of this lecture, you will be able to: 1) Explain the general mechanisms of prokaryotic gene regulation. 2) Describe the role of lactose in the induction of ! - Galactosidase in E. coli . 3) Design an experiment using indicator plates coated with X-Gal to assay ! -Galactosidase activity. 4) Explain how replica plating was used in a genetic screen to identify the lacZ , lacY , and lacI genes.
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1. A cell does not express all of its genes all of the time. ! Gene expression occurs when a gene product is actively being synthesized and used in a cell. Regulation of gene expression is critical to the efficient use of resources and thus survival. ! Cells are very selective about the genes they express, how strongly they are expressed, and when they are expressed. During the next two sections:
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Escherichia coli has served as an excellent model organism for the study of prokaryotic gene regulation. ! Like most bacteria, E. coli can use a wide array of carbohydrates to supply carbon and energy. Control of gene expression allows E. coli to respond to its environment and switch its use of sugars. ! Gene expression in bacteria was predicted to be
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This note was uploaded on 04/02/2012 for the course BIO 142 taught by Professor Escabar during the Spring '08 term at Emory.

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Lecture 16 - Biol142 Foundations in Modern Biology II...

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