bio142 quiz2 key

bio142 quiz2 key - Quiz #2 (Fri 114) NAME: DATE: 1. Name...

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Quiz #2 (Fri 114) NAME: DATE: 1. Name three critical factors that we need to consider when designing effective primers for PCR? (1.5 points) A. Primer length, longer has less efficient annealing, shorter has less specific annealing B. Melting temperature Tm C. Specificity D. Complementary primer sequence, primer should not anneal to itself or another primer E. G/C content, about 45-55%; more G/C causes increase in TM F. 3’ end sequence, adding a G or C to last 3 nucleotides increases efficiency 2. If you start with a single strand of DNA, how many copies do you have after 6 cycles? (1 point) (Please give the formula and the result.) 2 6 =64 3. Why do you boil your sample when you are isolating your DNA with 10% Chelex? (1 point) A. Its main purpose is to lyse the cells, open cells up to release DNA B. it can kill off bacteria that may have been with your cells, reduce the contamination of PCR 4. What are the 3 distinct steps of PCR and what is the temperature for each step? (3 points)
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This note was uploaded on 04/02/2012 for the course BIO 142L taught by Professor Conniearthur during the Spring '12 term at Emory.

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bio142 quiz2 key - Quiz #2 (Fri 114) NAME: DATE: 1. Name...

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